Reconstitution Guide (Research Use Only)
⚠️ Important: All peptides are provided as lyophilized powder. This guide is for laboratory research purposes only. Not for human use.
What You’ll Need
- Lyophilized peptide vial
- Sterile Bacteriostatic Water — We strongly recommend only using Hospira brand.
- Syringe Barrel (typically 3 mL)
- Reconstitution Needle (25g, 1.5” long)
- Alcohol Prep Pads
- Optional: Syringe Filter + New Sterile Vial (for extra peace of mind)
- Optional: Venting Needle (30g, 1” long) to make drawing easier after reconstitution
Need sterile supplies? Visit our Lab Supplies section.
Step-by-Step
- Check Your Vial — Confirm how many milligrams (mg) of peptide it contains.
- Clean Your Workspace — Wipe surfaces and vial tops with alcohol pads. You can also spray your supplies and work area with 70% alcohol.
- Calculate Amount of Bacteriostatic Water Needed — If you need any assistance, please feel free to message or email us.
• Example: If you want 2 mg/mL and your vial has 10 mg, you’ll add 5 mL of water.
• Formula: Volume = Mass ÷ Concentration.
- Add the Water Slowly — Aim the syringe down the inside wall of the vial. Don’t shake hard — gently swirl until it dissolves. <(Optional) Use a venting needle to relieve pressure as you add water.
- (Optional) — Push the solution through a sterile syringe filter into a new sterile vial.
- Write the Date — Mark the vial with the reconstitution date.
- Store Safely — Lyophilized vials are typically stable for years when stored properly (often in the freezer). Avoid repeated freeze–thaw cycles; split into smaller vials if needed.
Example Calculation
• Vial has: 10 mg
• Goal: 2 mg/mL
• Calculation: 10 ÷ 2 = 5 mL
• Result: 10 mg in 5 mL → final concentration 2 mg/mL
Notes & Tips
- Always work sterile.
- Don’t reuse needles or syringes.
- Dispose of materials safely.
- If unsure, filtering or venting adds extra confidence.
For Research Use Only. Not for Human Consumption.
