Reconstitution Guide (Research Use Only)
⚠️ Important: All products are provided as lyophilized powder. This guide is for laboratory research purposes only. Not for human use.
What You’ll Need
- Lyophilized vial
- Sterile Bacteriostatic Water — Hospira brand recommended
- Syringe barrel (typically 3 mL)
- Reconstitution needle (25g, 1.5”)
- Alcohol prep pads
- Optional: syringe filter + new sterile vial
- Optional: venting needle (30g, 1”)
Need sterile supplies? Visit our Lab Supplies section.
Step-by-Step
- Confirm vial amount — Verify how many milligrams (mg) are in the vial.
- Prep/Sterilize — Clean vial tops and workspace with alcohol.
- Draw bacteriostatic water — Use sterile syringe and needle to draw desired amount.
- Add water slowly — Aim down vial wall. Swirl/roll gently, do not shake hard.
- (Optional) — Filter solution into a new sterile vial.
- Label + store — Mark date and store refrigerated.
For Research Use Only.
Calculator
Use the inputs below to calculate concentration and draw volumes.
Reference: U-100 insulin syringes mark 100 units = 1.00 mL.
Common presets (tap to apply):
Concentration: —
Per Unit (U-100): —
“Per unit” = mg delivered per 1 unit marking on a U-100 syringe.
Units to mg Reference
units × mg/unit
| Insulin Units |
Amount (mg) |
| 10 units | — |
| 20 units | — |
| 25 units | — |
| 50 units | — |
| 100 units | — |
Note: Units are U-100 syringe volume markings.
Draw Volume Calculator
Enter a target amount (mg). We’ll calculate the draw volume based on your reconstitution inputs.
Draw: —
Volume: —
Calculated using: units = target mg ÷ (mg per unit)
Tip: If you change vial mg or mL above, this updates automatically.
Notes & Tips
- Always maintain sterile technique.
- Never reuse needles or syringes.
- Dispose of sharps safely.
- Filtering and venting can reduce contamination risk.
For Research Use Only. Not for Human Consumption.
